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肿瘤分子药物研究室
编辑:     发布日期:2017-03-31    来源:

一、团队简介

江南大学医学院肿瘤分子药物研究室长期致力于天然产物抗肿瘤效应和机制以及抗肿瘤药物手性中间体的制备等研究工作。主要研究方向包括(1)天然产物的结构解析及生物活性;(2)天然产物抗肿瘤机制;(3)新型酶基因的挖掘及理性改造;(4)功能性食品及药物手性中间体的生物制备。近5年来,本团队成员主持或参与国家自然基金6项,省市级以及企业委托项目30余项。在研究生和本科生科研创新方面成效显著,获得5项高校研究生科研创新计划项目和3项国家大学生创新计划项目以及“挑战杯”全国大学生课外学术科技作品竞赛国家二等奖。以第一作者/通讯作者发表SCI论文50余篇,其中影响因子大于3的9篇。授权发明专利4项。主编和参编论著及教材6部。本研究室拥有层次合理、训练有素、凝聚力强的科研队伍以及先进的仪器设备和和谐的文化氛围,现有教授1名,副教授1名,讲师1名,实验技术人员1名,每年招收博士、硕士研究生3-4名。

二、团队负责人简介

邬敏辰,江南大学医学院教授,博士生导师。主要从事分子生物学、药学及生物化工等的教学和科研工作。主要研究方向:(1)新型酶基因的挖掘及理性改造;(2)功能性食品及药物手性中间体的生物制备。主持和主要参与国家自然科学基金4项,完成国家、省、校级和企业委托项目30余项,指导国家大学生创新实验计划项目3项、江苏省研究生创新计划项目7项、江南大学博士研究生科学研究基金项目3项。主编和参编论著4部,发表学术论文200余篇,其中SCI收录论文40余篇。指导硕/博士研究生40余名。

三、团队成员简介

邓超,江南大学医学院副教授,硕士生导师。主要研究方向:天然多糖的免疫调节和抗肿瘤活性及其机制。相关研究成果在国内外学术期刊发表30余篇,其中以第一作者/通信作者发表SCI论文7篇,CSCD论文6篇。申请国家发明专利20项,授权发明专利4项。参与国家自然科学基金面上项目和青年基金项目2项,江苏省产学研项目和社会发展项目2项,国家和校级大学生创新项目3项。第十三届“挑战杯”全国大学生课外学术科技作品竞赛国家二等奖,江苏省一等奖指导教师。

王克伟,江南大学医学院讲师。主要研究方向:慢性病流行病学与时间序列模型的构建和应用研究,目前已发表论文10余篇,其中SCI一作4篇,中文一作3篇,SCI共同一作1篇,通讯作者1篇,参与国家自然科学基金面上项目1项,主持无锡市社会发展科技支撑项目资助1项。

四、团队代表性科研项目

序号

项目名称

项目来源

项目批准号

资助经费(万元)

项目起止期限

1

基于理性化设计的β-甘露聚糖酶分子定向改造

国家自然科学基金面上项目

31271811

77

2013.01~16.12

2

宇佐美曲霉木聚糖酶耐热性的分子改造

糖化学与生物技术教育部重点实验室开放课题

KLCCB-

KF201208

3

2012~2014

3

甲状旁腺激素二联体药物的开发

江苏省科技支撑项目

BE2010620

12

2010~2012

4

阿魏酸酯酶A的克隆、表达及基于理性设计的耐热性改造

江苏省普通高校研究生科研创新计划

CXZZ13_0757

1

2013.6~2014.6

5

宇佐美曲霉环氧化物水解酶对映选择性的定向改造及其应用

江苏省普通高校学术学位研究生创新计划

KYLX15_1151

1

2015.4~2016.6

6

酶法制备(S)-2-(4-硝基苯基)环氧乙烷的研究

国家级大学生创新创业训练计划

2015021Z

1

2015.5~2016.5

7

社区老年糖尿病患者自我管理系统的研发与应用

无锡市社会发展科技支撑项目

CSE31N1516

8

2015.7~2017.12

五、团队代表性科研成果

论文:

(1) Dong YH, Li JF, Hu D, Yin X, Wang CJ, Tang SH, Wu MC*. Replacing a piece of loop-structure in the substrate-binding groove of Aspergillus usamii β-mannanase, AuMan5A, to improve its enzymatic properties by rational design. Appl Microbiol Biotechnol, 2016, 100(9): 3989-3998. (IF=3.337)

(2) Hu D, Tang CD, Yang B, Liu JC, Yu T, Deng C, Wu MC*. Expression of a novel epoxide hydrolase of Aspergillus usamii E001 in Escherichia coli and its performance in resolution of racemic styrene oxide. J Ind Microbiol Biotechnol, 2015, 42(5): 671-680. (IF=2.439)

(3) Yin X, Hu D, Li JF, He Y, Zhu TD, Wu MC*. Contribution of disulfide bridges to the thermostability of a type A feruloyl esterase from Aspergillus usamii. PLoS ONE, 2015, 10(5): e0126864. (IF=3.234)

(4) Hu D, Ye HH, Wu MC*, Feng F, Zhu LJ, Yin X, Li JF. Chemoenzymatic preparation of (S)-p-nitrostyrene oxide from p-nitrophenacyl bromide by recombinant Escherichia coli cells expressing a novel halohydrin dehalogenase. Catalysis Communications, 2015, 69(1): 72-75. (IF=3.699)

(5) Yin X, Li JF, Wang CJ, Hu D, Wu Q, Gu Y, Wu MC*. Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis. Appl Microbiol Biotechnol, 2015, 99(23): 10047-10056. (IF= 3.337)

(6) Zhang HM, Li JF, Wang JQ, Yang YJ, Wu MC*. Determinants for the improved thermostability of a mesophilic family 11 xylanase predicted by computational methods. Biotechnol Biofuels, 2014, 7(1):3. (IF=6.221)

(7) Li JF, Wei XH, Tang CD, Wang JQ, Zhao M, Pang QF, Wu MC*. Directed modification of the Aspergillus usamii β-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis. J Ind Microbiol Biotechnol, 2014, 41(4): 693-700. (IF=2.505)

(8) Tan ZB, Li JF, Li XT, Gu Y, Wu MC*, Wu J, Wang JQ. A unique mono- and diacylglycerol lipase from Penicillium cyclopium: heterologous expression, biochemical characterization and molecular basis for its substrate selectivity. Plos One, 2014, 9(7): e102040. (IF=3.534)

(9) Zeng Y, Yin X, Wu MC*, Yu T, Feng F, Zhu TD, Pang QF. Expression of a novel feruloyl esterase from Aspergillus oryzae in Pichia pastoris with esterification activity. J Mol Catal B-Enzym, 2014, 110: 140–146. (IF=2.745)

(10) Wang JQ, Yin X, Wu MC*, Zhang HM, Gao SJ, Wei JT, Tang CD, Li JF. Expression of a family 10 xylanase gene from Aspergillus usamii E001 in Pichia pastoris and characterization of the recombinant enzyme. J Ind Microbiol Biotechnol, 2013, 40(1): 75-83. (IF=2.505)

(11) Gao SJ, Wang JQ, Wu MC*, Zhang HM, Yin X, Li JF. Engineering hyperthermostability into a mesophilic family 11 xylanase from Aspergillus oryzae by in silico design of N-terminus substitution. Biotechnol Bioeng, 2013, 110(4): 1028-1038. (IF=4.164)

(12) Tang CD, Li JF, Wei XH, Min R, Gao SJ, Wang JQ, Yin X, Wu MC*. Fusing a carbohydrate-binding module into the Aspergillus usamii β-mannanase to improve its thermostability and cellulose-binding capacity by in silico design. Plos One, 2013, 8(5): e64766. (IF=3.534)

(13) Gong YY, Yin X, Zhang HM, Wu MC*, Tang CD, Wang JQ, Pang QF. Cloning, expression of a feruloyl esterase from Aspergillus usamii E001 and its applicability in generating ferulic acid from wheat bran. J Ind Microbiol Biotechnol, 2013, 40(12): 1433-1441. (IF=2.505)

(14) Shi HL, Yin X, Wu MC*, Tang CD, Zhang HM, Li JF. Cloning and bioinformatics analysis of an endoglucanase gene (Aucel12A) from Aspergillus usamii and its functional expression in Pichia pastoris. J Ind Microbiol Biotechnol, 2012, 39(2): 347-357. (IF=2.321)

(15) Li JF, Zhao SG, Tang CD, Wang JQ, Wu MC*. Cloning and Functional Expression of an acidophilic β-mannanase gene (Anman5A) from Aspergillus niger LW-1 in Pichia pastoris. J Agric Food Chem, 2012, 60(3): 765-773. (IF=2.906)

(16) Wu MC*, Tang CD, Li JF, Zhang HM, Guo J. Bimutation breeding of Aspergillus niger strain for enhancing β-mannanase production by solid-state fermentation. Carbohydr Res, 2011, 346(14): 2149-2155. (IF=2.332)

(17) Deng C, Shang JY, Fu HT, Chen JX, Liu HY, Chen JH. Mechanism of the immunostimulatory activity by a polysaccharide from Dictyophora indusiata. Int J Biol Macromol, 2016, 91(10): 752-759. (IF=3.13)

(18) Deng C, Fu HT, Xu JJ, Shang JY, Cheng YM. Physiochemical and biological properties of phosphorylated polysaccharides from Dictyophora indusiata. Int J Biol Macromol, 2015, 72(1): 894-899. (IF=2.85)

(19) Deng C, Fu HT, Teng LP, Hu Z, Xu XF, Chen JH, Ren TL. Anti-tumor activity of the regenerated triple-helical polysaccharide from Dictyophora indusiata. Int J Biol Macromol, 2013, 61(10): 453-458. (IF=2.68)

(20) Deng C, Hu Z, Fu HT, Hu MH, Xu X, Chen JH. Chemical analysis and antioxidant activity in vitro of a β-d-glucan isolated from Dictyophora indusiata. Int J Biol Macromol, 2012, 51(1): 70-75. (IF=2.58)

(21) Wang KW, Shu ZK, Cai L, Wu JQ, Wei W. Assessment of the magnitude of contextual and individual demographic effects on diabetes mellitus and glucose intolerance in rural Southwest China: a multilevel analysis. Plos One, 2013, 8(7):374-374. (IF=3.75)

(22) Wang KW, Wu JQ, Zhao HX, Li YY, Zhao R, Zhou Y. Unmarried male migrants and sexual risk behavior: a cross-sectional study in Shanghai, China. Bmc Public Health, 2013, 13(1):1152-1152. (IF=2.21)

(23) Wu JQ, Wang KW (共同一作), Zhao R, Li YY, Zhou Y, Li YR, Ji HL, Ji M. Male rural-to-urban migrants and risky sexual behavior: a cross-sectional study in Shanghai, China. International Journal of Environmental Research & Public Health, 2014, 11(3):2846-2864. (IF=2.04)

(24) Wang KW, Wu GS, Li JP, Song WT. Role of vitamin d receptor gene cdx2, and apa1, polymorphisms in prostate cancer susceptibility: a meta-analysis. BMC Cancer, 2016, 16(1)1:9. (IF= 3.27)


专利:

(1)邬敏辰,李剑芳,张树飞,李江华,徐春梅,盛金萍,邓珊珊,方诗月.一种利用黑曲霉生产β-甘露聚糖酶的方法.授权号ZL 200710022406.X

(2)邬敏辰,李剑芳,朱劼,周晨妍,王瑾,李东峰,符丹丹,邓超.一种利用宇佐美曲霉产业化生产木聚糖酶的方法.授权号ZL 200710022405.5

(3)邓超,曹光群,陈敬华,程建青.茶树菇发酵美白液的制备方法.授权号ZL 201010195443.2

(4)邓超,王敏,陈敬华,滕丽萍.一种金耳发酵美白液的制备方法.授权号ZL 201210464470.4

(5)邓超,付海田,陈敬华.一种添加中药提取液的金耳深层发酵方法.授权号ZL 201310491993.2

(6)邓超,李英,陈丹霞.具有降血糖作用的保健食品及其制备方法.授权号ZL 201410351603.6


著作和教材:

(1) 《制药工程实验教程》人民卫生出版社,2010,参编

(2) 《医学微观形态学实验教程》化学工业出版社,2015,副主编


获奖:

(1)第十三届挑战杯全国大学生课外学术科技作品国家级二等奖指导教师

六、团队合影

合影.JPG

团队合影1

胡蝶.jpg

团队合影2

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关闭

技术支持:信息化建设与管理中心

地址:江苏省无锡市蠡湖大道1800号

邮编:214122

联系电话:0510-85328363